Journal of Molluscan Research, (JMR) Volume 8, 2022
Author: AJIBOYE*, O. O., ABIONA, J. A., ONAGBESAN, O. M., DUROTOYE, V. L.
ABSTRACT
A study was conducted on the development of methodologies for lethal and non-lethal haemolymph collection in giant African land snails (Archachatina marginata).
Twenty-seven (27) snails, were allocated into three groups (G1, G2 and G3) with weight ranges of 100 – 200g (G1); 201-300g (G2) and 301-400g (G3) with each having three replicates of 3 snails per each.
Parameters considered in this study were: Site of haemolymph collection (Buccal, foot and heart sinuses), live weight groups and volume of haemolymph collected.
Results showed that the site of haemolymph collection had no significant effect on haemolymph volume. Also, the volume of haemolymph collected from the three live weight groups was not significantly different (p>0.05).
Mortality only occurred among the group of snails in which haemolymph was collected via the heart. It is therefore recommended that haemolymph be collected using both buccal and foot sinuses in order to avoid snail mortality irrespective of the live weight.
Keyword: Haemolymph, Site of collection, Archachatina marginata, Giant African Land snail
